Abstract
1757
Objectives Real-time visualization of neuroinflammation in murine experimental autoimmune encephalomyelitis (EAE), a multiple sclerosis animal model, with 18F Fluoro-2-deoxy-D-glucose (FDG) animal Positron Emission Tomography (PET).
Methods EAE mice were induced in 10-week old male C57BJ 6 mice by administration of myelin oligodendrocyte glycoprotein (MOG35-55) peptide, tuberculosis (TB), Complete Freund’s adjuvant (CFA) and pertussis. The control group mice were also injected with all the above immune components except MOG35-55 peptide. Mice were scanned by F18-FDG-microPET at five time-points: before induction EAE; Day 2; day 9; Day 23; Day 35 after induction of EAE. Fluorine-18 sodium fluoride-PET was also performed among the same mice for skeleton anatomy correlation. Micro-CT database of the mice with similar body weight was also used for anatomy correlation purpose.
Results The FDG uptake levels of central nervous system (CNS) were sequenced as Day9 > Day 23 > Day 35 > Day 2 > Day 0. There was no significant CNS FDG-avid activity among the control group mice.
Conclusions Longitudinal 18F-FDG PET imaging could be applied to monitor the specific dynamic neuroinflammation process in the autoimmune disease in vivo. The peak neuroinflammation activity occurs a few days ahead of EAE onset. Then the FDG-avid activity in CNS decreases gradually. This dynamic finding is consistent with pro-inflammation cytokine profiles