Abstract
1626
Objectives: To study the effect of differentiation for Papillary thyroid carcinoma and Follicular thyroid carcinoma cell lines induced by Retinoic acid (RA) and Trichostatin A (TSA) using thyroglobulin (TG) as the target of tumor marker
Methods: After the cell lines treated with different RA , TSA and combined RA and TSA, TG concentration in these cell lines culture was investigated with vitro electrochemistry luminescence method
Results: In the concentration range of experiments, it was shown that the concentration of TG excreted from of K1 and FTC-133 was increased with the concentration level of RA, which is concentration - dependent on the RA. The low to medium range of TSA concentration will help the increase of the TG excreted from the cell, while it decreased with high TSA concentration. The TG level from DTC was significantly increased under combined-effect of the RA and low concentration of the TSA, while only appearing slightly increase with medium concentration of TSA and RA. It was also found that the TG level of K1 and FTC-133 was time-dependent on the RA and TSA concentration, either in independent acting or in combined acting. TG concentration of the cell treated by the drugs was highest after 48 hour
Conclusions: The RA, TSA and combined RA and TSA had the differentiation effect of inducing the cell lines, there were differentiation increment to DTC cell induced by combined low concentration of TSA and RA, but only had weak effect induced by combined medium concentration of TSA and RA
- Society of Nuclear Medicine, Inc.