Investigations of a 99mTc-Labeled Bacteriophage as a Potential Infection-Specific Imaging Agent
- Mary Rusckowski, PhD,
- Suresh Gupta, PhD,
- Guozheng Liu, PhD,
- Shuping Dou, BS and
- Donald J Hnatowich, PhD
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FIGURE 1.Histogram of activity remaining at the origin with ITLC in acetone and paper chromatography in saline of labeled phage in serum or buffer over time.
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FIGURE 2.In vitro measure of 99mTc-MAG3-phage binding to bacteria. Percentage of added activity bound is shown for E. coli 2537 (black bars), E. coli 25922 (white bars), and S. aureus (hatched bars).
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FIGURE 3.Percentage of added activity remaining in the supernatant following centrifugation after incubation of 99mTc-phage with E. coli 2537 (black circles) and in the same supernatant after 0.2 μm filtration (white circles). The decrease in activity after filtration demonstrates that the activity was associated with particles.
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FIGURE 4.Reversed-phase C-18 HPLC radiochromatograms. Shown is the 0.2 μm filtrate of the 30 min supernatant after incubation of 99mTc-labeled phage with E. coli 2537. Included as standards are 99mTcO4− and 99mTc-MAG3.
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FIGURE 5.Radioactivity levels (percentage injected dose [% ID] per organ) of 99mTc-labeled phage in tissues of normal mice at 0.5, 3, 6, and 24 h after administration. Liver, the highest organ of accumulation, is shown on its own scale.
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FIGURE 6.For the 3 bacterial preparations, comparison of activity (percentage injected dose [% ID]) in the infected thigh (black bars) and inflamed thigh (white bars) of mice, with the significance between the two indicated.
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FIGURE 7.For the 3 bacterial preparations, whole-body images at 3 h after administration of 99mTc-MAG3 phage to mice with an infection (left panels) or inflammation (right panels) in the target thigh (arrows).
