Uptake of 18F-Labeled 6-Fluoro-6-Deoxy-D-Glucose by Skeletal Muscle Is Responsive to Insulin Stimulation
Chandra Spring-Robinson 1,
Visvanathan Chandramouli 2,
William C. Schumann 2,
Peter F. Faulhaber 3,
Yanming Wang 4,
Chunying Wu 4,
Faramarz Ismail-Beigi 2,
and
Raymond F. Muzic Jr.5*
1 Department of Biomedical Engineering, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio
2 Department of Medicine, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio
3 Department of Radiology, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio
4 Department of Radiology, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio; Case Center for Imaging Research, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio
5 Department of Biomedical Engineering, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio; Department of Radiology, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio; Case Center for Imaging Research, University Hospitals Case Medical Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio
* To whom correspondence should be addressed. E-mail: raymond.muzic{at}case.edu.
 |
Abstract |
|---|
We are developing a methodology for the noninvasive imaging of glucose transport in vivo with PET and 18F-labeled 6-fluoro-6-deoxy-D-glucose (18F-6FDG), a tracer that is transported but not phosphorylated. To validate the method, we evaluated the biodistribution of 18F-6FDG to test whether it is consistent with the known properties of glucose transport, particularly with regard to insulin stimulation of glucose transport. Methods: Under glucose clamp conditions, rats were imaged at the baseline and under conditions of hyperinsulinemia. Results: The images showed that the radioactivity concentration in skeletal muscle was higher in the presence of insulin than at the baseline. We also found evidence that the metabolism of 18F-6FDG was negligible in several tissues. Conclusion: 18F-6FDG is a valid tracer that can be used in in vivo transport studies. PET studies performed under glucose clamp conditions demonstrated that the uptake of nonphosphorylated glucose transport tracer 18F-6FDG is sensitive to insulin stimulation.
Key Words:
glucose transport, radiopharmaceutical, PET, glucose clamp
