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FIGURE 4. Relationship between p53 gene expression and accumulation of radioiodine in SK-Hep1 cells expressing p53RE-hNIS (SK-Hep1p53NIS) after adriamycin treatment. (A) Western blot analysis and radioiodine uptake. After treatment of SK-Hep1p53NIS cells with adriamycin, 125I uptake of cells increased with increasing adriamycin dose. Levels of expression of total p53 and activated p53 (p53 Ser15) proteins increased in dose-dependent manner in SK-Hep1p53NIS cells. This increase in cells was completely inhibited by KClO4. (B) Scintigraphic images of endogenous p53 activation. Tumor xenografts were implanted in nude mice at 4 different sites: SK-Hep1 (a, 1 x 107, negative control); SK-Hep1p53NIS (b, 5 x 106; c, 1 x 107; d, 2 x 107). Scintigraphic images of 99mTc were obtained before and after adriamycin treatment in same mouse. Scintigraphy showed higher radioactivity in test tumors (SK-Hep1p53NIS) after adriamycin treatment than in control tumor (SK-Hep1). (C) Radioactivity ratio of SK-Hep1p53NIS to SK-Hep1 in mice before and after adriamycin treatment. Significantly higher uptake was observed in SK-Hep1p53NIS tumors than in SK-Hep1 tumor after adriamycin treatment or in nontreated SK-Hep1p53NIS tumors.
