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FIGURE 2. Components of TF2 anti-CEA x anti-HSG bs-mAb. (A) C-DDD2-Fab-hMN-14 was formed by linking a cysteine-modified peptide sequence (DDD2), which is composed of amino acids 1–44 of human RII
, to the carboxyl-terminal end of the Fd chain via a 14-residue flexible peptide linker. (B) Antihapten binding arm was made by linking the 17-residue amino-acid sequence derived from AKAP-IS, a synthetic peptide optimized for RII-selective binding, to the carboxyl end of the Fd via a 15-residue flexible peptide linker with the addition of cysteine residues to both the amino- and carboxyl-terminal ends of AD2. TF2 is the anti-CEA x anti-HSG recombinant humanized protein formed by combining A and B.
