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FIGURE 5. (A) Doxycycline-mediated inhibition of HSV1-TK messenger RNA for NG4TL4/tet-off-HSV1-TK cells, NG4TL4/tet-off-mNLSHSV1-TK cells, and NG4TL4/tet-off-mNLSHSV1-TK:dMODC cells. (B) Doxycycline-mediated inhibition of HSV1-TK enzyme activity for NG4TL4/tet-off-HSV1-TK cells, NG4TL4/tet-off-mNLSHSV1-TK cells, and NG4TL4/tet-off-mNLSHSV1-TK:dMODC cells. Stably transformed cell lines were treated with 0, 0.01, 0.1, 1, and 10 ng of doxycycline per milliliter. After doxycycline treatment, changes in messenger RNA levels and enzyme activity of HSV1-TK were assayed at various time points using real-time PCR and in vitro assay for HSV1-TK enzyme activity, respectively. Bars represent mean ± SD of 3 independent experiments.
