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FIGURE 3. (A) Fluorescence stabilities of HSV1-TK:GFP and mNLSHSV1-TK:dMODC:GFP in presence of cycloheximide, and with or without MG-132 (26S proteasome inhibitor), were examined with fluorescence microscope. (B) TK enzyme stabilities of HSV1-TK:GFP and mNLSHSV1-TK:dMODC:GFP in presence of cycloheximide, and with or without MG-132 (26S proteasome inhibitor), were measured by HSV1-TK enzyme activity assay. NG4TL4 cells were transfected with vectors expressing these 2 proteins. After 24 h, transfected cells were treated with 100 mg of cycloheximide per milliliter for 6 h, with or without 40 µM MG-132. Fluorescence stabilities and TK enzyme stability of these fusion proteins were examined with fluorescence microscope and HSV1-TK enzyme activity assay, respectively.
