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FIGURE 3. (A and B) In vitro binding kinetics of FROP-1. FRO82-2 and MCF-7 cells were grown for 24 h. 125I-FROP-1 was added and incubated for 5, 10, 15, 30, 60, 120, 180, and 240 min without (A) or with (B) 1% BSA. (C) In vitro competition assay with FROP-1 using various competitor concentrations. FRO82-2 and MCF-7 cells were grown for 24 h. Unlabeled FROP-1 in concentrations ranging from 10–4 M to 10–7 M were added to cells immediately before 125I-FROP-1 and incubated for 1 h. Experiments were performed in triplicate; SDs are shown.
