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FIGURE 1. Development of reporter system and reporter cells. (A) Structure of pQcmv-hNET-IRES-GFP vector. (B) Fluorescence-activated cell sorting (FACS) analyses of C6 (1), COS-7 (2), and Jurkat (3) wild-type and pQcmv-hNET-IRES-GFP transduced cells after FACS. LTR = long terminal repeat; dLTR = LTR with deleted promoter sequence; M1 = range of GFP expression; FLT-1 = fluorescent channel 1.
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