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FIGURE 4. Comparison of in vitro GLP-1 receptor autoradiography of lung in rat and human. (A and C) Hemotoxylin- and eosin-stained (HE) tissue sections show the following: (A) rat lung with alveolar septa (stars) and large blood vessels (arrows) and (C) human lung with alveolar septa (stars) and small blood vessels (arrows). Bars = 1 mm. (B and D) Autoradiograms show total binding of 125I-GLP-1(7–36)amide. (B) Rat lung with very strong and diffuse binding to alveolar septa (stars) and no binding to large blood vessels (arrows). (D) Human lung with topically restricted binding, partly to small blood vessels (arrows) but not to alveolar septa (stars). Complete displacement of 125I-GLP-1(7–36)amide by 100 nM unlabeled (cold) GLP-1(7–36)amide provides proof of specific GLP-1 receptor binding (not shown).
