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FIGURE 2. In vitro receptor autoradiography on human pancreas. (A) Immunohistochemistry for synaptophysin staining islets (arrowheads) surrounded by acini. Bar = 1 mm. (B) Autoradiogram shows total binding of ¹²⁵I-GLP-1(7–36)amide: strong binding to islets, weak binding to acini. (C) Autoradiogram shows nonspecific binding of ¹²⁵I-GLP-1(7–36)amide in presence of 100 nM unlabeled (cold) GLP-1(7–36)amide.

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