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FIGURE 6. CA-IX and pimonidazole staining on FSA and R3230Ac tumor sections. (A and B) 64Cu-ATSM autoradiography (AR) and CA-IX staining (green) on same FSA tumor section (in same tumor as shown in Fig. 5, FSA panel). CA-IX staining shows a hypoxia distribution that is consistent with EF5 staining. (C and D) 64Cu-ATSM autoradiography and pimonidazole staining (orange) with Hoechst 33342 vascular perfusion marker (blue) illustrate low correlation between 64Cu-ATSM and this hypoxia marker in FSA. (E and F) 64Cu-ATSM autoradiography and pimonidazole staining in R3230Ac tumor. Close correlation can be seen between 64Cu-ATSM uptake and pimonidazole staining.
