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FIGURE 5. Comparisons between 64Cu-ATSM uptake and hypoxia measured by immunostaining in R3230Ac and FSA. Close correlation between 64Cu-ATSM uptake and EF5-stained hypoxic area was observed in R3230Ac tumor (left), whereas no correlation was found in FSA tumor (right). Images include 64Cu-ATSM microPET image, autoradiography (AR) section from same tumor, EF5 and Hoechst immunostaining from adjacent section, fused image from autoradiography and EF5 images, H&E staining, and correlation plot between autoradiography and EF5 staining images. EF5-stained hypoxic area is indicated by orange, perfused vessels are marked by blue fluorescent Hoechst 33342 dye, and 64Cu-ATSM distribution in AR is indicated by green in fused image. In FSA, a large amount of 64Cu-ATSM accumulated in well-perfused areas, which are indicated in Hoechst perfusion image. The spatial correlation between autoradiography and EF5 staining images in this specific FSA tumor is 0.05, whereas the spatial correlation is 0.78 in the shown R3230Ac tumor.
