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FIGURE 4. Typical immunostaining of EF5 binding in tumor tissues (R3230Ac). Dual fluorescence images (A) are overlaid to show distribution of hypoxia relative to vasculature. Hypoxic tissue is indicated by EF5 staining in orange. Vasculature is indicated by the Hoechst 33342 perfusion marker in blue. (B) A whole tumor section staining image was generated by stitching together images from microscope stage scans.

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