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FIGURE 1. Image thresholding in area fraction measurement. (A and B) Original 64Cu-ATSM autoradiography image and EF5 fluorescent staining image. (C and D) Corresponding histograms for above images. The Otsu automatic thresholding method was used to determine the threshold based on histograms. Pixels with intensity higher than the threshold (in white color) were treated as positive uptake or staining area. (E and F) Segmented images using the Otsu threshold for autoradiography and EF5 staining image. Area in white is regarded as 64Cu-ATSM uptake area or positive staining area.
