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FIGURE 4. Binding and competition of 125I-labeled p160 and 125I-ß-Ala-p160-8-2. (A) Specific binding of 125I-p160 and 125I-ß-Ala-p160-8-2 to WAC 2 cells. As competitors, unlabeled p160 and ß-Ala-p160-8-2 were used at concentration of 5 x 10–5 mol/L. Incubation was performed for 1 h at 37°C. (B) Time kinetics of 125I-ß-Ala-p160-8-2 binding to WAC 2. (C) Unlabeled ß-Ala-p160-8-2 was incubated in human serum, and aliquots were removed for subsequent analysis at indicated time points. Experiments A and B were performed in triplicate; SD is shown.
