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FIGURE 1. Binding and competition of 125I-labeled p160. (A) Specific binding of 125I-p160 to neuroblastoma WAC 2 cells. Nonspecific binding was determined in presence of unlabeled p160 (10–4 mol/L). The peptides octreotide and D-p160 were used at same concentration (10–4 mol/L) as negative control competitors. (B) Binding of 125I-p160 with and without excess of unlabeled peptide (10–4 mol/L) in WAC 2 and HUVEC cells. Incubation was performed for 1 h at 37°C. (C) Competition for bound 125I-p160 by unlabeled p160 peptide. WAC 2 cells were incubated with 1–2 x 106 cpm radioligand and increasing concentrations of unlabeled p160 peptide. (D) In vitro cell accumulation of 125I-p160 in WAC 2 cells as function of time. Incubation was performed for periods from 5 to 180 min. All experiments were performed in triplicate; SD is shown.
