Click on image to view larger version.
FIGURE 3. (A) Ten-micron-thick frozen sections of rabbit atherosclerotic aorta stained with 50 µg/mL (panel a), 10 µg/mL (panel b), 5 µg/mL (panel c), and 1 µg/mL (panel d) of bispecific antibody counterstained with 5.5 HRP polymer, 150 µg/mL Z2D3 F(ab')2 (panel e), or 50 µg/mL (panel f), 10 µg/mL (panel g), 5 µg/mL (panel h), and 1 µg/mL of Z2D3 F(ab')2 (panel i) counterstained with HRP-conjugated secondary antibody. (B) Fluorescent (panels a, c, and e) micrographs show presence or absence of rhodamine fluorescence of surrogate antigen-coated beads with bispecific antibody (panel a), empty beads with bispecific antibody (panel c), or surrogate antigen-coated beads with NSB control (panel e) targeted with rhodamine-labeled DTPA–Suc-PL14.6 kDa. Corresponding light micrographs are shown in panels b, d, and f, respectively.
