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FIGURE 2. (A) Immunoreactivity of bispecific antibody and Z2D3 F(ab')2 binding to surrogate antigen-coated microtiter wells by ELISA. (B) Immunoreactivity of bispecific antibody and anti-DTPA F(ab')2 binding to DTPA-BSA antigen-coated microtiter wells by ELISA. (C) Demonstration of signal enhancement with bispecific antibody and DTPA-HRP–Suc-PL46 kDa with 5.5 and 9 HRP per polymer relative to Z2D3 F(ab')2 and secondary HRP-conjugated rabbit antihuman IgG antibody (RAH). Negative control is anti-DTPA F(ab')2. OD = optical density.
