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FIGURE 3. sst3 internalization is induced by various agonists and is efficiently abolished by sst3-specific antagonist sst3-ODN-8. HEK-sst3 cells were treated either with vehicle (no peptide [A]) or with various agonists at concentrations inducing submaximal internalization effect (100 nmol/L [B–D]). Cells treated with same agonists at same concentrations as in B–D but in presence of excess of specific sst3-antagonist sst3-ODN-8 (5 µmol/L [F–H]). Effect of antagonist alone (50 µmol/L [E]) is also illustrated. After incubation with peptides, cells were processed for immunocytochemistry. Clear perinuclear staining is detectable for all agonists tested. This staining is efficiently abolished by 50-fold excess of antagonist sst3-ODN-8. Antagonist alone has no effect on internalization.
