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FIGURE 4. TUNEL assay of liver slices from 3 different imaging studies. Liver slices were subjected to TUNEL assay, and number of positive nuclei was counted (1525 fields per slide). Untreated liver slices typically showed 1%2% positive nuclei per field, whereas cycloheximide treatment resulted in 10%16% positive nuclei. Panels show data from study in Figure 2A (2-h animal was not imaged) (A), data from separate study (B), and data from study in Figure 2B (90-min animal was not imaged) (C). Error bars refer to SD of mean.