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FIGURE 3. Correlation of apoptotic morphology and cycloheximide treatment conditions. (A) H&E-stained slides were made from paraffin blocks of liver samples from rats treated with cycloheximide (5 mg/kg) for times indicated. Slides were scored for percentage of apoptotic cells, scoring minimum of 15 fields per slide. Each data point represents single animal; error bars represent SD of mean value of all fields read from same slice. (B) H&E slides were similarly prepared and scored from rats treated for 90 min with the indicated concentrations of cycloheximide, before injection of 18F-annexin V. Because these rats were also imaged for 2 h after injection of radiotracer, total duration of exposure to cycloheximide was 210 min. Numbers of animals per data point were 4 for no Rx, 2 for 2 mg/kg, and 4 for 5 mg/kg; error bars refer to SD of mean values.
