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FIGURE 2. Immunohistochemical analyses and fluorescence microscopy of sections from rat brain gliomas 10 d after implantation of tumor cells in right caudate putamen. (A) GFAP immunoreactivity of reactive astrocytes is increased in immediate vicinity of tumor (Tu), as indicated by arrowheads. Scale bar = 1 mm. (B) OX-42 immunoreactivity as a marker of activated microglia spreads far beyond peritumoral zone into cortical and limbic regions, as indicated by arrowheads. Scale bar = 1 mm. (C) Confocal fluorescence images of GFAP (green) and A1AR (red) in tumor periphery indicate that a subpopulation of GFAP-immunopositive cells expresses A1ARs (yellow). Tumor cells in solid tumor mass as well as in invasion zone (arrowheads) are also immunoreactive for A1ARs (red). Scale bar =200 µm. (D) High magnification of C. Subpopulation of astrocytes closely associated with a vessel (V) in tumor margin is immunoreactive for both GFAP (green) and A1ARs (red). Cellular colocalization is indicated by yellow. A1AR immunoreactivity (red) is also found next to cells staining for GFAP. Scale bar = 25 µm. (E) Confocal microscopy of neuronal marker SMI311 (green) and A1AR (red) indicating cellular colocalization of both immunostains (yellow). Scale bar = 50 µm.
