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FIGURE 1. Autoradiographic studies. (A) Quantification of data from F98 glioma ex vivo animal experiments reveals that receptor densities in tumor margin, tumor (total), and control region (contralateral temporal cortex [Temp. ctx.]) differ significantly (P < 0.01) (asterisks). WW = wet weight. Error bars indicate SDs. (B) Two representative ex vivo autoradiographs display intense accumulation of 3H-CPFPX at periphery of tumor. Left panel displays solid tumor; right panel displays tumor with central necrosis. Ex vivo experiments were performed 10 d after implantation of F98 tumor cells in right caudate putamen. (C) Same sections as in B. Ex vivo autoradiographs obtained with 18F-FET delineate solid tumor mass. For direct reading of absolute receptor density and amino acid (AA) uptake, see black–white scales between sections. (D) Histograms of 3H-CPFPX (red line), 18F-FET (green line), and Nissl stain (blue line) taken from levels indicated by respective colored lines in B, C, and E. A1AR density peaks at margin of solid tumor mass. OD = optical density. (E) Nissl stains of 2 sections depicted in B and C.
