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FIGURE 1. Imaging of hNIS gene transfer using 125I- and autoradiography. (A) Upper panel demonstrates the high correlation between average pixel intensity per well and Ad-hNIS viral titer. Bold line denotes linear regression from 0 to 20 MOI. Lower panel shows increasing signal of autoradiographs with increasing Ad-hNIS titer. (B) Upper panel demonstrates the high correlation that exists between average pixel intensity per well and activity of added 125I-. Line denotes linear regression of pixel intensity per well vs. 125I- activity. Lower panel shows increasing signal intensity with increasing 125I- activity in MB-435 cells infected with 25 MOI of Ad-hNIS. Data are expressed as the mean ± SD of 3 replicates and are representative of at least 3 separate experiments.
