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FIGURE 4. Native PAGE of sera recovered from normal and AP-immunized mice reacted ex vivo with 125I-AP. Mice were given 0.1 mg AP through intradermal or intravenous route once daily for 3 d. Two weeks after last injection, mice were euthanized. Sera collected from mice were incubated with 125I-AP overnight and examined by electrophoresis on native PAGE gel. Lanes: 1, control, C57BL/6 mouse serum; 2, control, Swiss Albino mouse serum; 3, immunized mouse serum, Swiss Albino, intradermal; 4, immunized mouse serum, Swiss Albino, intradermal; 5, immunized mouse serum, Swiss Albino, intravenous; 6, immunized mouse serum, Swiss Albino, intravenous.
