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FIGURE 5. (A) Quantitative autoradiography (QAR) of 20-µm frozen sections of brain taken from 3 littermate control mice (top row) or 3 transgenic mice (bottom row) that were killed 6 h after single intravenous injection of 125I-HD-PNA-8D3 conjugate. (B) Film autoradiography of 20-µm frozen sections of brain taken from 3 transgenic mice that were killed 6 h after single intravenous injection of 125I-HD-PNA-8D3 conjugate (bottom row), and 3 transgenic mice that were killed 6 h after single intravenous injection of 125I-luc-PNA-8D3 conjugate (top row). (C) QAR of 20-µm-thick 125I-microscale standard strips is shown in inset. Integrated density for each standard is plotted versus known radioactivity for standard. (D) Integrated density obtained from scanning autoradiograms of brain sections taken from either littermate control mice or transgenic mice (A) was converted into measurements of organ radioactivity (mBq [µCi]/g) based on standard curve (C). Data indicate there is more than 3-fold increase in sequestration of brain radioactivity at 6 h after intravenous injection in transgenic mice compared with littermate control mice. Data are mean ± SE, n = 3 mice per group.
