FIGURE 2. Hematoxylin–eosin staining (A) and autoradiograms (B) from 1 experiment. Each tissue section was mounted on a slide. Sections were selected to include sites of laser treatment. (A and B) Ten sections displayed in 2 rows. (A) Regions of laser channels are marked by blue circles. (B) Phosphor screen captured image of perfusion tracer (99mTc-sestamibi) is displayed in gray scale and superimposed are results of 2 subtraction processes. Red pixels represent relatively higher MIBG than MIBI and green pixels represent relatively lower MIBG than MIBI when compared with remaining myocardium (gray). Yellow circles correspond to locations of laser holes identified from hematoxylin–eosin sections. Only 1 laser site with confluent green pixels showed reduced MIBG (second section from right in second row).
