FIGURE 3. (A) HPLC after incubation of ¹⁸F-FECh with choline kinase and with choline oxidase. No-carrier-added ¹⁸F-FECh chloride was incubated with yeast choline kinase and 15 µmol ATP or with bacterial choline oxidase. From incubated specimen, methanol-water–soluble component was separated and fractionated on cation- and anion-exchange HPLC. ¹⁸F radioactivity was measured in each fraction. (B) HPLC after incubation of ¹⁸F-FECh with choline kinase and -³²P-ATP. No-carrier-added ¹⁸F-FECh chloride (approximately 0.005 µmol) was incubated with yeast choline kinase and 0.01 µmol -³²P-ATP. From incubated specimen, methanol-water–soluble component was separated and fractionated on anion-exchange HPLC. ¹⁸F and ³²P radioactivities were measured in each fraction. kcpm = kilocounts per minute.

Return to article