FIGURE 3. HPLC visualization of temporary coating of antibody with IODO-GEN and subsequent removal of coating by ascorbic acid through simultaneous UV detection at 315 nm, second absorption maximum of IODO-GEN (UV 2), and, at 280 nm, absorption maximum of MAb (UV 1). Shown are native MAb (A), coated MAb (B), and MAb with coating removed (C). Conditions are described in text. Large peak at 40 min (C) is from ascorbic acid; minor peaks (at 16 and 39 min) in chromatograms (A and B) at 315 nm are solvent peaks.
