FIGURE 4. Intracellular distribution of 125I-, 211At-, 111In-, 205,6Bi-, and 203Pb-labeled T101 in PBMNC. PBMNC incubated with radiolabeled T101 were suspended in TES buffer and disrupted. Cell nuclei and unbroken cells were removed by centrifugation at 250g. Supernatants were applied to Percoll/TES buffer and ultracentrifuged at 4°C for 60 min at 20,000g. Serial 0.5 mL fractions were collected from top and counted for radioactivity. Early fractions represent activity on cell surface and late fractions represent activity in lysosomes (fraction 14). Location of lysosome fraction was confirmed using enzyme marker ß-galactosidase (data not shown).
