HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH RSS TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JNM Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zhao, M. Articles by Bugenhagen, S. Search for Related Content PubMed PubMed Citation Articles by Zhao, M. Articles by Bugenhagen, S.

^99mTc-Labeled Duramycin as a Novel Phosphatidylethanolamine-Binding Molecular Probe

Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin

View larger version (18K): [in this window] [in a new window]   FIGURE 1.  (A) Diagram illustrating primary structure of duramycin with cross-linking bridges. (B and C) Radio-HPLC chromatograms of 99mTc-duramycin before and after purification are shown in B and C, respectively. Low, but significant, level of 99mTc-pertechnetate is present before purification with retention time of 2 min. (D) Representative competition curve of 3 independent experiments. 99mTc-duramycin binding of apoptotic cells is competitively diminished by presence of PtdE-containing liposomes (•) but not other phospholipid species, including PtdC (), PtdG (), or PtdS (). cpm = counts per minute.

View larger version (13K): [in this window] [in a new window]   FIGURE 2.  (A) Blood clearance of 99mTc-duramycin in healthy rats (n = 3). (B–E) Radio-HPLC chromatograms of 99mTc-duramycin standard (B), serum at 1 min after injection (C), serum at 5 min after injection (D), and urine at 60 min after injection (E).

View larger version (68K): [in this window] [in a new window]   FIGURE 3.  (A) Dynamic planar imaging of 99mTc-duramycin distribution in healthy rat. Note rapid renal clearance of radiotracer and low hepatic uptake. (B) Whole-body dynamic planar imaging of 99mTc-duramycin uptake in rat with acute myocardial infarction. (C) Non–color-enhanced, raw counts of static planar images of sham-operated rat (left) and infarcted rat (right) acquired at 120 min after intravenous injection of 99mTc-duramycin. Infarct site is marked by arrows. In autoradiography, radioactivity uptake in myocardium colocalizes with infarct with excellent infarct-to-noninfarct ratio (inset). B = bladder; K = kidney.