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microPET-Based Biodistribution of Quantum Dots in Living Mice

¹ Departments of Radiology and Bioengineering, Bio-X Program, Molecular Imaging Program at Stanford (MIPS), Stanford University, Stanford, California; ² California NanoSystems Institute (CNSI), UCLA School of Medicine, Los Angeles, California; ³ Department of Chemistry and Biochemistry, UCLA School of Medicine, Los Angeles, California; and ⁴ Department of Molecular & Medical Pharmacology, Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, California

View larger version (20K): [in this window] [in a new window]   FIGURE 1.  Ex vivo biodistribution of 64Cu-labeled, pegylated QD525 (A) and QD800 (B) as measured by well counting. Radiolabeled QD (1.85 MBq) were injected into tail vein of nude mice. Groups of mice (n = 3) were sacrificed at 10 min, 30 min, 1.5 h, 4.5 h, 12 h, and 36 h, and organs were harvested and counted. Mean and SD of %ID/g have been corrected for physical decay of isotope.

View larger version (13K): [in this window] [in a new window]   FIGURE 2.  Image-based in vivo biodistribution of 64Cu-labeled, pegylated QD525 (A and B) and QD800 (C and D) as measured by image ROI analysis of microPET datasets. During dynamic image acquisition, 5.55 MBq of radiolabeled QD were injected into tail vein of nude mice (n = 4). Images were acquired dynamically in 10-s frames for the first 10 min and one 5-min frame thereafter (A and C; error bars are omitted for better visibility). Mice were reimaged in 5-min static acquisitions at 1, 3, 6, 12, and 36 h (B and D), coregistered with microCT images, and AMIDE image analysis software was used to obtain organ activity information. Mean and SD of %ID/g have not been corrected for physical decay.

View larger version (100K): [in this window] [in a new window]   FIGURE 3.  In vivo PET images of mice injected with 64Cu (first column), 64Cu-DOTA (second column), QD525 (third column), QD525PEG (fourth column), QD800 (fifth column), or QD800PEG (sixth column). During dynamic image acquisition, 5.55 MBq of the respective agent were injected into tail vein of nude mice. Images were acquired dynamically in 10-s frames for the first 10 min and one 5-min frame thereafter. Coronal (upper row), sagittal (middle row), and transverse (lower row) slices of a 5-min frame from 10 to 15 min after injection are shown.

View larger version (11K): [in this window] [in a new window]   FIGURE 4.  Image-based in vivo biodistribution of 64Cu-labeled, unpegylated QD525 (A and B) and QD800 (C and D) as measured by ROI analysis of microPET datasets. During dynamic image acquisition, 5.55 MBq of radiolabeled QD were injected into tail vein of nude mice (n = 4). Images were acquired dynamically in 10-s frames for the first 10 min and one 5-min frame thereafter (A and C; error bars are omitted for better visibility). Mice were reimaged in 5-min static acquisitions at 1, 3, 6, 12, and 36 h (B and D), coregistered with microCT images, and AMIDE image analysis software was used to obtain organ activity information. Mean and SD of %ID/g have not been corrected for physical decay.

View larger version (11K): [in this window] [in a new window]   FIGURE 5.  Image-based in vivo biodistribution of 64Cu (A and B) and DOTA-64Cu (C and D) as measured by ROI analysis of PET datasets. During dynamic image acquisition, 5.55 MBq of the respective tracer were injected into tail vein of nude mice (n = 2). Images were acquired dynamically in 10-s frames for the first 10 min and one 5-min frame thereafter (A and C; error bars are omitted for better visibility). Mice were reimaged in 5-min static acquisitions at 1, 3, 6, 12, and 36 h (B and D), coregistered with microCT images, and AMIDE image analysis software was used to obtain organ activity information. Mean and SD of %ID/g have not been corrected for physical decay.