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Reduced Iodide Transport (Stunning) and DNA Synthesis in Thyrocytes Exposed to Low Absorbed Doses from ¹³¹I In Vitro

¹ Department of Radiation Physics, Göteborg University, Göteborg, Sweden; and ² Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden

View larger version (12K): [in this window] [in a new window]   FIGURE 1.  Bicameral culture system. Pig thyrocytes were grown on micropore filter (pore size, 0.4 µm) that divided well into apical and basal compartments corresponding to follicular lumen and extrafollicular space, respectively.

View larger version (8K): [in this window] [in a new window]   FIGURE 2.  Experimental protocols for confluent cells (A) and subconfluent cells (B). Cell culturing was started at time 0.

View larger version (8K): [in this window] [in a new window]   FIGURE 3.  125I– transport through 131I-irradiated cells relative to nonirradiated cells. Relative 125I– transport is ratio of 125I– transported through irradiated cells to 125I– transported through nonirradiated control cells at different absorbed doses. * = data that were statistically significantly different from control data (P < 0.05). Data are given as mean ± SEM (n = 6).

View larger version (10K): [in this window] [in a new window]   FIGURE 4.  Relative 3H-thymidine incorporation normalized to first time point measured and total amount of DNA per sample in nonirradiated porcine thyrocytes at different times after start of culturing (day 0). 3H-Thymidine incorporation shows how cells initially proliferated, incorporating large amounts of thymidine, until they became confluent at about day 6. Total DNA content increased until confluence was reached. Data are given as mean ± SEM (n = 3).

View larger version (10K): [in this window] [in a new window]   FIGURE 5.  Effect of 131I irradiation on subsequent 3H-thymidine incorporation. Results are given as 3H-thymidine incorporation in irradiated cultures relative to that in nonirradiated cultures. Hypersensitivity was obtained at 0.01 and 0.1 Gy (see inset). * = data that were statistically significantly different from control data (P < 0.05). Data are given as mean ± SEM (n = 3–21).

View larger version (7K): [in this window] [in a new window]   FIGURE 6.  Total DNA per sample in 131I-irradiated cell cultures relative to that in nonirradiated cultures vs. absorbed doses to cells. * = data that were statistically significantly different from control data (P < 0.05). Data are given as mean ± SEM (n = 3).