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Stunning and Its Effect on 3H-FDG Uptake and Key Gene Expression in Breast Cancer Cells Undergoing Chemotherapy

James M. Engles, BS1, Shelley A. Quarless, MD, PhD1, Elizabeth Mambo, PhD2, Takayoshi Ishimori, MD, PhD1, Steve Y. Cho, MD1 and Richard L. Wahl, MD1

1 Russell H. Morgan Department of Radiology and Radiological Science, Division of Nuclear Medicine, Johns Hopkins University, Baltimore, Maryland; and 2 Department of Otolaryngology, Division of Head and Neck Cancer Research, Johns Hopkins University, Baltimore, Maryland


Figure 1
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  		FIGURE 1.  Actual cell number as calculated from standard curve.

 

Figure 2
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  		FIGURE 2.  3H-FDG uptake per cell (A) and per well (B) in untreated and treated MCF-7 cells.

 

Figure 3
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  		FIGURE 3.  Comparison of glut-1 mRNA, HKII mRNA, 3H-FDG per cell, and MTT results in untreated MCF-7 cells.

 

Figure 4
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  		FIGURE 4.  Comparison of glut-1 mRNA, HKII mRNA, 3H-FDG per cell, and MTT results in DOX-treated MCF-7 cells.

 

Figure 5
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  		FIGURE 5.  Comparison of Glut-1 mRNA, HKII mRNA, 3H-FDG per cell, and MTT results in 5FU-treated MCF7 cells.

 

Figure 6
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  		FIGURE 6.  Results of regression analysis using real-time mRNA data and 3H-FDG uptake per cell: untreated vs. glut-1 (A), untreated vs. HKII (B), DOX vs. glut-1 (C), DOX vs. HKII (D), 5FU vs. glut-1 (E), and 5FU vs. HKII (F). y-Axis represents percentage of baseline expression of mRNA; x-axis represents percentage of baseline 3H-FDG uptake.

 

Figure 7
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  		FIGURE 7.  Western blot analysis of total protein extractions from untreated and DOX- and 5FU-treated MCF-7 cells. Comparable amounts of total protein are loaded in each lane, as shown by ß-actin. HKII protein rises in untreated cells and falls in treated cells. Glut-1 protein levels change less than does mRNA. UNT = untreated.

 




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