HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JNM Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schmidt, K. Articles by Kinscherf, R. Search for Related Content PubMed PubMed Citation Articles by Schmidt, K. Articles by Kinscherf, R.

Angiostatin Overexpression in Morris Hepatoma Results in Decreased Tumor Growth but Increased Perfusion and Vascularization

¹ Department of Anatomy and Cell Biology III, University of Heidelberg, INF 307, Heidelberg, Germany; ² Clinical Cooperation Unit Nuclear Medicine, DKFZ and University of Heidelberg, INF 280, Heidelberg, Germany; ³ Department of Nuclear Medicine, University of Heidelberg, INF 400, Heidelberg, Germany; ⁴ Department of Immunology, University of Rostock, Rostock, Germany; ⁵ Department of Biophysics and Medical Radiation Physics, DKFZ, INF 280, Heidelberg, Germany; ⁶ Department of Internal Medicine III, INF 410, University of Heidelberg, Germany; and ⁷ Department of Radiopharmaceutical Chemistry, German Cancer Research Centre, Heidelberg, Germany

View larger version (63K): [in a new window]   FIGURE 1.  Expression of hANG in clones of MH3924A. (A) Northern blot. (B) Western blot. Representative blots are shown. -Tubulin or ß-actin was used as internal control. bp = base pairs.

View larger version (15K): [in a new window]   FIGURE 2.  Proliferation and apoptosis of HUVECs cocultured with WT-MH3924A or hANG-MH3924A. (A) Proliferation (acid-soluble and acid-insoluble fractions of thymidine uptake). (B) Apoptosis. Values are shown as mean + SEM (*P < 0.05 vs. WT-MH3924A).

View larger version (16K): [in a new window]   FIGURE 3.  Tumor growth of WT-MH3924A and hANG-MH3924A tumors until 40 d after inoculation. Values are shown as mean ± SEM (***P < 0.001; **P < 0.01; vs. WT-MH3924A).

View larger version (11K): [in a new window]   FIGURE 4.  Histomorphometric analyses of apoptotic and proliferative cells (as indicated by TUNEL technique [A] or PCNA staining [B]) in WT-MH3924A and hANG-MH3924A tumors. Cell density (nuclei/mm2) or percentage of positive cells was quantified by analyzing 5 ROIs (n = 6 each group). Values are shown as mean + SEM (***P < 0.001; **P < 0.01 vs. WT-MH3924A).

View larger version (19K): [in a new window]   FIGURE 5.  H215O PET. Tumor perfusion (K1, k2, DV, and VB values) measured in WT-MH3924A (n = 7) and hANG-MH3924A (n = 8) of ACI rats; 2D acquisition (1-tissue compartment model). hANG-expressing tumors showed a significantly increased K1 (A), whereas k2 remained unchanged (B). The fractional volume of distribution (DV) increased significantly in hANG-MH3924A (C), whereas the vascular fraction (VB) showed no difference (D).

View larger version (9K): [in a new window]   FIGURE 6.  68Ga-DOTA-albumin PET: Tumor blood volume of WT-MH3924A (n = 5) and hANG-MH3924A (n = 8) in ACI rats; 2D acquisition, SUVs were calculated. hANG-overexpressing tumors demonstrated a significant increase in tumor blood volume in comparison with WT-MH3924A.

View larger version (14K): [in a new window]   FIGURE 7.  Immunohistomorphometric analysis of vascularization in Morris hepatomas. WT-MH3924A and hANG-MH3924A were stained with -actin or CD31 antibodies (each group, n = 6). Control immunoglobulin (data not shown) was used as negative controls. Immunoreactive areas were quantified by analyzing 5 hot spots per tumor. Center = center of tumor. Values are shown as mean + SEM (**P < 0.01 vs. WT-MH3924A).