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Iodide Kinetics and Dosimetry In Vivo After Transfer of the Human Sodium Iodide Symporter Gene in Rat Thyroid Carcinoma Cells

¹ Department of Nuclear Medicine, University of Heidelberg, Heidelberg, Germany
² Clinical Cooperation Unit Nuclear Medicine, Deutsche Krebsforschungszentrum, Heidelberg, Germany
³ Department of Anatomy and Cell Biology III, University of Heidelberg, Heidelberg, Germany
⁴ Department of Radiation Protection, Deutsche Krebsforschungszentrum, Heidelberg, Germany
⁵ Department of Radiochemistry and Radiopharmacology, Deutsche Krebsforschungszentrum, Heidelberg, Germany

View larger version (13K): [in a new window]   FIGURE 1. Time dependence of 125I– uptake in wild-type rat thyroid carcinoma cells and in the hNIS-expressing cell line L2hNIShyg3. Values are mean and SD (n = 3).

View larger version (13K): [in a new window]   FIGURE 2. Effect of DIDS, FCCP, sodium perchlorate (SP), and ouabain (ouab) on 125I– uptake in wild-type rat thyroid carcinoma cells and in the hNIS-expressing cell line L2hNIShyg3 after 1 h of incubation. Values are mean and SD (n = 3). con = control.

View larger version (10K): [in a new window]   FIGURE 3. Iodide efflux from L2hNIShyg3 cells after 1 h of incubation with Na125I with and without 300 µmol of DIDS per liter. Values are mean and SD (n = 3).

View larger version (76K): [in a new window]   FIGURE 4. Scintigraphic images of tumor-bearing nude mice with subcutaneously transplanted hNIS-expressing (right thigh) or wild-type (left thigh) thyroid carcinoma cells at 2 h (A), 6 h (B), 24 h (C), and 48 h (D) after injection of 131I–.

View larger version (11K): [in a new window]   FIGURE 5. 131I– uptake (kBq/g) in hNIS-expressing and wild-type thyroid carcinomas after administration of 1,650 MBq/m2 at different times after radiotracer application in rats. Values are mean and SDs (n = 4).