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¹¹¹In-Labeled CD34+ Hematopoietic Progenitor Cells in a Rat Myocardial Infarction Model

¹ Department of Nuclear Medicine, University Hospital Kiel, Kiel, Germany
² Department of Molecular Cardiology, Internal Medicine IV, University of Frankfurt, Frankfurt, Germany
³ Department of Pediatric Hematology and Oncology, University of Frankfurt, Frankfurt, Germany

View larger version (14K): [in a new window]   FIGURE 1. Trypan-blue staining. Values are given as percentages of dead cells of controls as determined by counting 5 microscopic random fields of view (n = 3 each). Significant differences were found at 48 and 96 h after radiolabeling.

View larger version (158K): [in a new window]   FIGURE 2. Pinhole images of nude rats without (A–C) or with (D–F) myocardial infarction after injection of 111In-oxine–labeled HPCs into the left ventricular cavity. At 1 h after injection, a high lung uptake was visible on the spot images of the chest and abdomen (A and D), but was no longer detectable on images at 24 (B and E) and 96 (C and F) h after injection. The spot images of the chest and abdomen after 96 h clearly showed significant differences in cardiac uptake between infarcted (F) and sham-operated (C) animals.

View larger version (18K): [in a new window]   FIGURE 3. Distribution pattern of specific activity (megabecquerel per gram of tissue per megabecquerel of injected activity) in different tissues after left intraventricular administration of 111In-oxine–labeled HPCs in infarcted or sham-operated nude rats. Data are given as mean ± SD (n = 4 each). Significant differences between organs of sham-operated and infarcted animals were observed only in the heart.

View larger version (60K): [in a new window]   FIGURE 4. CMFDA cell-tracker–labeled human CD34+ HPCs (green) were identified in the infarct border zone delineated by damaged -sarcomeric actinin-positive cardiac myocytes (red). Nuclei were stained with TO-PRO-3 (blue).