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Characterization of ¹⁸F-FDG Uptake in Human Endothelial Cells In Vitro

Department of Nuclear Medicine, Laboratory of Molecular Imaging, Friedrich-Alexander University, Erlangen, Germany

View larger version (31K): [in a new window]   FIGURE 1. Radio-TLC of intracellular metabolites of 18F-FDG in HUVECs. Radio-TLC was performed on silica gel–coated plastic sheets with acetonitrile/tetrabutylammonium hydroxide, 9.5 mmol/L, 8:2 (v/v), as eluant. 18F-FDG-1,6-dP = 18F-FDG-1,6-diphosphate; 18F-FDG-6P = 18F-FDG-6-phosphate.

View larger version (15K): [in a new window]   FIGURE 2. Influence of phlorizin on 18F-FDG uptake by HUVECs. Values are mean ± SD of a single experiment performed in triplicate (n = 3). One similar experiment yielded qualitatively identical results. c = concentration.

View larger version (15K): [in a new window]   FIGURE 3. Inhibition of 18F-FDG uptake in HUVECs by cytochalasin B. Cytochalasin B competitively inhibits 18F-FDG uptake with an IC50 of 0.16 µmol/L. Values are mean ± COV (% of mean) of 2 independent experiments performed in triplicate (n = 6). c = concentration.

View larger version (16K): [in a new window]   FIGURE 4. Influence of deprivation of glucose and sodium depletion on 18F-FDG uptake in HUVECs. Glucose deprivation significantly enhances 18F-FDG uptake by a factor of 2.7 (P < 0.02). Values are mean ± SD of a single experiment performed in triplicate (n = 3). Two similar experiments yielded qualitatively identical results. t = time.

View larger version (125K): [in a new window]   FIGURE 5. Immunocytochemical staining of HUVECs. Monoclonal anti-VEGF receptor-2 (KDR) was used as the primary antibody. (A) Negative control. (B) Primary antibody.

View larger version (10K): [in a new window]   FIGURE 6. Influence of VEGF stimulation on 18F-FDG uptake in HUVECs. HUVECs from the fourth passage were treated with VEGF (50 ng/mL, 2 h) and incubated with 18F-FDG for 3 h at 4°C in PBS. Values are mean ± SD of 2 independent experiments (n = 6; P < 0.003).

View larger version (12K): [in a new window]   FIGURE 7. 18F-FDG uptake in HUVECs, GLIOs, and human monocytes differentiated to macrophages (HMMs). HUVECs were from the fourth passage, HMMs differentiated to macrophages during 14 d of culture, and GLIOs were incubated for 3 h with 18F-FDG at 4°C in PBS. Values are mean ± SD of multiple independent experiments: HUVECs, n = 22; GLIOs, n = 6; HMMs, n = 6.