PET of Cardiac Transgene Expression: Comparison of 2 Approaches Based on Herpesviral Thymidine Kinase Reporter Gene
Masao Miyagawa, MD, PhD1,
Martina Anton, PhD2,
Roland Haubner, PhD1,
Marcus V. Simoes, MD, PhD1,
Christian Städele, DVM2,3,
Wolf Erhardt, DVM2,
Sybille Reder, MT1,
Terry Lehner, BSc1,
Bettina Wagner, DVM1,
Steffi Noll, PhD4,
Bernhard Noll, PhD4,
Michaela Grote, PhD4,
Sanjiv S. Gambhir, MD5,
Bernd Gansbacher, MD2,
Markus Schwaiger, MD1 and
Frank M. Bengel, MD1
1 Nuklearmedizinische Klinik und Poliklinik, Technische Universität München, Munich, Germany
2 Institut für Experimentelle Onkologie und Therapieforschung, Technische Universität München, Munich, Germany
3 Medizinische Klinik I, Technische Universität München, Munich, Germany
4 Institute for Bioinorganic and Radiopharmaceutical Chemistry, Forschungszentrum Rossendorf, Germany
5 Department of Radiology and Bio-X Program, Stanford University, Stanford, California
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FIGURE 1. (A and B) PET coronal slices of representative rats at 20–40 min and at 90–120 min after intravenous injection of 124I-FIAU (A) and 18F-FHBG (B), obtained 2 d after intramyocardial injection of Adtk (A) or Adsr39tk (B). (C and D) Cardiac tracer uptake over the entire 2 h after application of radiolabeled probes. Values are mean ± SD in groups after intramyocardial injections with Adtk or negative control (AdLacZ) for 124I-FIAU (C) and with Adsr39tk or AdLacZ for 18F-FHBG (D).
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FIGURE 2. Kinetics of radiolabeled probes in the liver, 124I-FIAU (A) and 18F-FHBG (B). Values are mean ± SD for each group.
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FIGURE 3. (A and B) PET slices of myocardial perfusion and early and late reporter probe distribution in pig hearts, 2 d after intramyocardial injection of Adtk (imaged by 124I-FIAU) (A) or Adsr39tk (imaged by 18F-FHBG) (B) into basal anterolateral wall. Shown are representative horizontal long-axis slices (top) and short-axis slices (bottom). (C and D) Values for the count ratio of virus injection area relative to remote myocardium over the entire 2 h after application of radiolabeled probes. Values are mean ± SD for 124I-FIAU (C) and 18F-FHBG (D). LA = left atrium; RA = right atrium; LV = left ventricle; RV = right ventricle.
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FIGURE 4. (A and B) Comparison of in vivo and ex vivo PET images of pig hearts. Shown are short-axis slices of a representative animal that was imaged using 124I-FIAU after intramyocardial injection of AdNull into basal and Adtk into apical anterolateral walls (A) (arrows mark injection site) and of another animal that was imaged using 18F-FHBG after injection of AdNull into basal and Adsr39tk into apical anterolateral walls (B). (C and D) Correlation plots for segmental ex vivo tracer concentration in all animals and early myocardial in vivo 124I-FIAU uptake (C) or late myocardial in vivo 18F-FHBG uptake (D).
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Copyright © 2004 by the Society of Nuclear Medicine.
