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Reagents and Methods for PET Using Bispecific Antibody Pretargeting and 68Ga-Radiolabeled Bivalent Hapten-Peptide-Chelate Conjugates

Gary L. Griffiths, PhD1, Chien-Hsing Chang, PhD1, William J. McBride, PhD1, Edmund A. Rossi, PhD1, Agatha Sheerin, BS1, German R. Tejada, BS1, Habibe Karacay, PhD2, Robert M. Sharkey, PhD2, Ivan D. Horak, MD1, Hans J. Hansen, PhD1 and David M. Goldenberg, ScD, MD2

1 Immunomedics, Inc., Morris Plains, New Jersey; IBC Pharmaceuticals, Inc., Morris Plains, New Jersey
2 Garden State Cancer Center, Belleville, New Jersey



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FIGURE 1. Dicistronic expression cassette for production of BS1.5H in E. coli.

 


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FIGURE 2. SE-HPLC analyses of 68Ga-IMP 241 after mixing with 50x molar excess of hMN-14 x 679 bsAb (top), and 67Ga-IMP 241 after a 4-h incubation in human serum and mixing with 50x molar excess of anti-HSG antibody 679 IgG (bottom).

 





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