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Stunning of Iodide Transport by ¹³¹I Irradiation in Cultured Thyroid Epithelial Cells

¹ Department of Radiation Physics, Sahlgrenska University Hospital, Göteborgs Universitet, Göteborg, Sweden
² Institute of Anatomy and Cell Biology, Göteborgs Universitet, Göteborg, Sweden
³ Department of Oncology, Sahlgrenska University Hospital, Göteborgs Universitet, Göteborg, Sweden
⁴ Department of Surgery, Sahlgrenska University Hospital, Göteborgs Universitet, Göteborg, Sweden
⁵ Department of Internal Medicine, Sahlgrenska University Hospital, Göteborgs Universitet, Göteborg, Sweden

View larger version (31K): [in a new window]   FIGURE 1. Transwell bicameral culture chamber system. Porcine thyroid cells form continuous monolayer epithelium on microporous filter, which constitutes bottom of Transwell insert. Monolayer is continuous and thus separates chamber into lower (basal) and upper (apical) compartment. Insert that holds filter is placed at fixed distance of 1 mm from base of basal well. Filter area, 0.33 cm2; filter height, 10 µm; filter pore size, 0.4 µm.

View larger version (24K): [in a new window]   FIGURE 2. Protocol for thyroid cell culture treatments (open arrows) and radioiodide transport analysis (filled arrows). Time scale indicates number of days after starting primary culture.

View larger version (13K): [in a new window]   FIGURE 3. Redistribution of 131I between basal and apical media of filter-cultured porcine thyrocytes during irradiation period (Dc = 15 Gy). Equal concentrations of 131I were present initially at both sides of cell monolayer. Results are presented as mean ± SEM (n = 6).

View larger version (13K): [in a new window]   FIGURE 4. Effect of 131I irradiation (Dc = 80 Gy) and equimolar concentrations of stable iodide (10-8 mol/L) on subsequent (3 d later) basal-to-apical 125I transport. Results are given as 125I activity transported into apical medium at indicated times relative to activity added to basal medium at time = 0. Values obtained are expressed as percentage of total activity present basally at start of experiment. Results are expressed as mean ± SEM (n = 3).

View larger version (12K): [in a new window]   FIGURE 5. Effect of different absorbed doses on iodide transport in 131I irradiated cultures. Results are presented as relative amount of 125I activity transported from basal to apical medium during 30 min. Values are given as mean ± SEM (n = 4).

View larger version (14K): [in a new window]   FIGURE 6. Effect of perchlorate (ClO4-) on iodide transport in 131I-irradiated cultures. Perchlorate (1 mmol/L) was present in apical and basal media during exposure to 131I (corresponding to Dc = 30 Gy without perchlorate) for 48 h. 125I transport was evaluated 3 d later. 131I-induced stunning of 125I transport was 90% when perchlorate was not present during irradiation. Results are expressed as mean ± SEM (n = 3).