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A 3-Step Pretargeting Strategy to Image Infection

Department of Nuclear Medicine, University Hospital Nijmegen, Nijmegen, The Netherlands

View larger version (27K): [in a new window]   FIGURE 1. Scintigraphic images of rats with S. aureus calf muscle infections. Rats received 0.66 nmol (100 µg) anti-DTPA antibody intravenously. Four hours later, 0.3 nmol (20 µg) BSA-DTPA-In was injected as blocking agent, and 15 min thereafter, rats received 111In-DTPA, 111In-DTPA-Lys, or 111In-diDTPA (8 pmol per rat, 4 MBq per rat). Images were acquired 2 h after injection of radiolabel.

View larger version (23K): [in a new window]   FIGURE 2. Scintigraphic images of rats with S. aureus calf muscle infections. Rats received 300 µg (2 nmol) anti-DTPA antibody intravenously. Four hours later, each group of rats received BSA-DTPA-In (0–0.3, 1, and 3) to block anti-DTPA antibody activity in circulation. Fifteen minutes thereafter, rats received 111In-diDTPA (8 pmol per rat, 4 MBq per rat). Images were acquired 2 h after injection of radiolabel.

View larger version (25K): [in a new window]   FIGURE 3. Scintigraphic images of rats with S. aureus calf muscle infections. Rats received 300 µg (2 nmol) anti-DTPA antibody intravenously. Four hours later, rats received 1 nmol BSA-DTPA-In as blocking agent. Fifteen minutes thereafter, each group of rats received 111In-diDTPA (1.6, 8, and 40 pmol per rat, respectively; 4 MBq per rat). Images were acquired 2 h after injection of radiolabel.

View larger version (31K): [in a new window]   FIGURE 4. Scintigraphic images of rats with S. aureus calf muscle infections. Rats received 300 µg (2 nmol) anti-DTPA antibody intravenously. Rats received 1 nmol BSA-DTPA-In as blocking agent 1, 4, and 24 h later. Fifteen minutes thereafter, each group of rats received 111In-diDTPA (8 pmol per rat, 4 MBq per rat). Images were acquired 2 h after injection of radiolabel.

View larger version (35K): [in a new window]   FIGURE 5. Scintigraphic images of rats with S. aureus calf muscle infections. One group of rats was imaged according to optimized 3-step approach (2 nmol anti-DTPA antibody; 4 h later, 1 nmol BSA-DTPA-In; 5 min later, 40 pmol 111In-diDTPA). Other group of rats received 111In-IgG (4 MBq, 0.66 nmol). Both groups of rats were imaged 5 h after first intravenous injection.

View larger version (44K): [in a new window]   FIGURE 6. Scintigraphic images of rats with S. aureus calf muscle infections. Both groups received 2 nmol anti-DTPA antibody. Four hours later, anti-DTPA antibody activity in circulation was blocked with 1 nmol BSA-DTPA-In. Five minutes thereafter, one group of rats received 40 pmol 111In-diDTPA (4 MBq), whereas other group received 40 pmol 99mTc-diDTPA (5 MBq). Images were acquired 2 h after injection of radiolabel.