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Early Detection of Oleic Acid-Induced Lung Injury in Rats Using ¹¹¹In-Labeled Anti-Rat Intercellular Adhesion Molecule-1

Departments of Diagnostic Imaging and Therapeutics, Medicine, Surgery, and Pharmacology, University of Connecticut Health Center, Farmington; and Department of Radiology, VA Medical Center, Newington, Connecticut

View larger version (21K): [in a new window]   FIGURE 1. Experimental time line for injection of 111In-labeled radiopharmaceuticals (solid arrow), injury with OA or sham injury (dotted arrow), and imaging or biodistribution (solid arrow) of rats. Only 2 injury times, 4 and 24 h, are shown for clarity.

View larger version (13K): [in a new window]   FIGURE 2. Effect of time after OA injury on lung uptake of 111In-RSA and 111In-nmIgG. Data are given as percentage change compared with uninjured lung using %ID/O values (#, P < 0.05; *, P < 0.001).

View larger version (12K): [in a new window]   FIGURE 3. Effect of time after OA injury on lung uptake of 111In-PMNs and 111In-aICAM-1. Data are given as percentage change compared with uninjured lung using %ID/O values (#, P < 0.05; *, P < 0.001).

View larger version (24K): [in a new window]   FIGURE 4. Effect of time after OA injury on L/B (A) and L/H (B) for 111In-PMNs, 111In-aICAM-1, and 111In-nmIgG. Data were computed using %ID/O values (*, P < 0.001; +, 0.05 < P < 0.1; #, P < 0.05).

View larger version (95K): [in a new window]   FIGURE 5. Pinhole scintigrams of upper anterior torso of uninjured rats and rats at 4 and 24 h after OA injury injected with 111In-PMNs, 111In-aICAM-1, or 111In-nmIgG. Animals were imaged at 24 h after injection with GE gamma camera (General Electric, Milwaukee, WI).

View larger version (87K): [in a new window]   FIGURE 6. Expression of ICAM-1 immunofluorescence in cryostat sections of OA-injured and uninjured rat lung. Sections were processed for immunofluorescence using aICAM-1 as first antibody in lung from uninjured rat (A), rat injured at 4 h (C), and 24 h before kill (E). For control samples, no aICAM-1 was used as first antibody in lung from uninjured rat (B), rat injured at 4 h (D), and 24 h before kill (F). Rhodamine-conjugated goat antimouse IgG was used as second antibody in all sections (magnification, x788).