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^99mTc-Labeled Antimicrobial Peptides for Detection of Bacterial and Candida albicans Infections

Departments of Radiology and Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands; Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università degli Studi di Pisa, Pisa, Italy; and Centro de Investigaciones Nucleares and Cátedra de Radioquímica, Montevideo, Uruguay

View larger version (48K): [in a new window]   FIGURE 1. Typical scintigrams of 99mTc-labeled UBI 29–41 (A), hLF 1–11 (B), ciprofloxacin (C), and human polyclonal IgG (D) 1 h after injection into mice having thigh muscles infected with S. aureus.

View larger version (22K): [in a new window]   FIGURE 2. Accumulation of 99mTc-labeled antimicrobial peptide UBI 29–41 in thigh muscles of mice infected with MRSA (white bars), K. pneumoniae (square-hatched bars), or fluconazole-resistant C. albicans (black bars). Furthermore, mice were injected intramuscularly with LPS (diagonally hatched bars) or heat-killed bacteria (horizontally hatched bars) 18 h before administration of tracer. Each symbol at each time point represents mean ± SEM T/NT of at least 12 animals obtained from three independent experimental settings.

View larger version (33K): [in a new window]   FIGURE 3. Accumulation of 99mTc-labeled UBI 29–41 (white bars), UBI 18–35 (square-hatched bars), hLF 1–11 (horizontally hatched bars), ciprofloxacin (black bars), and IgG (checkered bars) in thigh muscles of mice infected 18 h earlier with fluconazole-resistant C. albicans. Each symbol at each time point represents mean ± SEM T/NT of at least six animals obtained from three independent experimental settings.

View larger version (53K): [in a new window]   FIGURE 4. Typical scintigrams of 99mTc-labeled UBI 29–41 (A), hLF 1–11 (B), ciprofloxacin (C), and human polyclonal IgG (D) 1 h after injection into mice having thigh muscles injected with heat-killed S. aureus.

View larger version (25K): [in a new window]   FIGURE 5. (A) Typical scintigram of 99mTc-labeled UBI 29–41 1 h after injection into rabbits with thigh muscles infected with S. aureus. (B) Biodistribution of 99mTc-labeled UBI 29–41 1 h after injection with lower contrast.

View larger version (22K): [in a new window]   FIGURE 6. Accumulation of 99mTc-labeled UBI 29–41 in thigh muscles of rabbits injected with MRSA (white bars), K. pneumoniae (square-hatched bars), heat-killed MRSA (diagonally hatched bars), heat-killed K. pneumoniae (horizontally hatched bars), or 50 mg LPS (black bars). Each symbol at each time point represents mean ± SEM T/NT of at least three animals obtained from independent experimental settings.