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In Vitro and In Vivo Tracer Characteristics of an Established Multidrug-Resistant Human Colon Cancer Cell Line

Departments of Neuroanatomy and Nuclear Medicine, University Hospital Eppendorf, Hamburg, Germany

View larger version (159K): [in a new window]   FIGURE 1. In vitro characteristics of HT-29par (A and C) and HT-29mdr1 (B and D) cell lines. Immunohistochemic visualization of P-gp expression: absence of label in HT-29par (A) and positive staining in HT-29mdr1 (B) cell lines. Functional assessment of P-gp was performed by Rhodamine 123 accumulation (C and D). Note bright fluorescence in HT-29par (C) cells in contrast to weak fluorescence in HT-29mdr1 cells (D) caused by increased efflux. Original magnification, x450.

View larger version (11K): [in a new window]   FIGURE 2. Uptake of 99mTc-MIBI and 201TlCl in HT-29par (black columns) and HT-29mdr1 (white columns) cells in percentage tracer in external medium. Data represent mean ± SD of nine cell suspensions each.

View larger version (90K): [in a new window]   FIGURE 3. Planar scintigraphic images from posterior view obtained 15 min after injection of 99mTc-MIBI in SCID mice carrying HT-29par (A) or HT-29mdr1 (B) xenografts between scapulae. Imaging of SCID mice carrying HT-29mdr1 tumors was repeated 60 min after injection (C). Upper threshold of color table was set to five times average tracer uptake in background. Physiologic tracer uptake is shown in liver, urinary bladder, and intestine. Tumors are not visible.

View larger version (88K): [in a new window]   FIGURE 4. Maximum intensity projections from posterior view computed from transverse tomographic PET images acquired 50 min after injection of FDG in SCID mice carrying HT-29par (A) or HT-29mdr1 (B) xenografts between scapulae. Uptake of glucose analog was increased significantly in all HT-29par tumors and in six of nine HT-29mdr1 tumors (arrows).

View larger version (168K): [in a new window]   FIGURE 5. Visualization of HT-29par tumor vasculature basement membranes by antilaminin immunohistochemistry. Tumors grown in SCID mice show numerous blood vessels in their periphery (arrowheads) but very few in their center (arrow). Original magnification, x450.

View larger version (100K): [in a new window]   FIGURE 6. Immunohistochemic demonstration of P-gp expression in xenografts grown in SCID mice. Note absence of label in HT-29par (A) and positive staining in HT-29mdr1 (B) tumors. Original magnification, x450.

View larger version (105K): [in a new window]   FIGURE 7. Electron micrographs of HT-29par xenografted tumor cells (A) and SCID mouse hepatocyte (B). Note paucity of mitochondria (asterisks) in HT-29par tumor cells and their abundance in SCID mouse hepatocytes. Original magnification, x18,000.