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Interaction of Sodium Ion With Serum Protein^1,2

Hines and Chicago, Illinois

ABSTRACT

A continuously monitored perfusion-dialysis system has been previously described by the authors (1) permitting the determination of the rate of diffusion of labeled sodium from a dialysis cell. This system has been employed to study the interaction between sodium ion and serum protein. In a cylinderical dialysis cell of 3.17 mm radius, the elution rate (t₀) of sodium ion is constant. With addition of serum proteins to the solution in the cell, an initial elution rate (t₁) is observed equal to t₀, followed by a slower rate (t₂). The ratio of t₂/t₁ is 1.35; this ratio is unchanged by altering temperature, pH or added potassium. Changing protein concentration results in equivocal change in ratio. Reduction of the fluid thickness to 1.15 mm or less results in the elimination of t₂ in the presence of serum protein and t₁ is equal to t₀. This phenomenon is interpreted as a disproportion of the frictional impedence to diffusion by the diffusional forces acting over short distances and more significantly to the rate limiting characteristics of the membrane due to increased membrane friction to diffusion when compared to the lesser frictional properties of water solutions of various viscosity.

FOOTNOTES

¹ From the Radioisotope Service, Veterans Administration Hospital, Hines, Illinois, Loyola University Stritch School of Medicine, Hines, Illinois, and the University of Illinois College of Medicine, Chicago, Illinois.

² This study was supported in part by U. S. Public Health Service Grant No. AMO6847 National Institute of Health, Bethesda, Maryland.