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Coated Charcoal Assay of Plasma Iron Binding Capacity and Iron Using Radioisotope Dilution and Hemoglobin-Coated Charcoal^1,2,

New York, New York

ABSTRACT

Using radioisotope dilution, and hemoglobin-coated charcoal for batch separation, plasma iron may be measured. From 0.5 ml of plasma, native transfersin-bound iron is released at pH 5.8 (by adding 0.5 ml of pH 5.3 buffer to the plasma), following which 3 µg labeled iron is added. After subsequent addition of buffer to raise the pH to 7.4 and thus allow rebinding of a portion of the pool of radiodiluted iron by transferrin, iron-transferrin complex is separated from excess free iron by hemoglobin-coated charcoal and the plasma iron level computed by an appropriate formula. This method provides a model for the assay of similar constituents in biologic fluids, in which the binder for the agent is naturally present in the fluid under assay.

FOOTNOTES

¹ This work was supported in part by Research Grants AM 01063, AM 09062, AM09564 from the National Institute of Arthritis and Metabolic Diseases of the USPHS, by the World Health Organization, and by the Albert A. List, Frederick Machlin and Anna Ruth Lowenberg Funds.

² Presented in part at the Fifth Annual Meeting of the American Society for Clinical Nutrition, Atlantic City, New Jersey, May 1, 1965 (1).

³ Recipient of Career Scientist Award I-435 from the Health Research Council of the City of New York.

⁴ Current Address: Medical Research Laboratory, Clinical Investigations Branch, United States Army Edgewood Arsenal, Edgewood Arsenal, Maryland. Formerly, Research Assistant, The Department of Hematology, The Mount Sinai Hospital, New York.

⁵ Current Address: Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. Formerly, Research Fellow of the World Health Organization at The Mount Sinai Hospital, New York.

⁶ Current Address: Department of Medicine, New York Medical College, New York. Formerly, Assistant Attending Hematologist, The Mount Sinai Hospital, New York.

⁷ Jointiy affiliated with The Mount Sinai Hospital of the City of New York and with the Department of Mathematics of C. W. Post College, Long Island University, Brookville, New York.

⁸ The Department of Hematology, The Mount Sinai Hospital and School of Medicine, New York City, N. Y.