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Endogenous Production of Carbon-14 Labeled Carbon Monoxide: An In Vivo Technique for the Study of Heme Catabolism¹

Berkeley, California

ABSTRACT

A method is presented for the separation, detection, and quantitation of endogenously produced carbon-14 carbon monoxide in the rat, following injection of glycine-2-¹⁴C. In this method, respiratory ¹⁴CO₂, the only significant breath contaminant, is removed with a sodalime absorber. The remaining breath activity, due primarily, if not entirely, to ¹⁴CO, oxidized to ¹⁴CO₂ by Hopcalite, absorbed in an ethanolamine-containing solution, and counted by liquid scintillation. Standard ¹⁴CO and ¹⁴CO₂ gases, as well as animal experimentation, confirm this method's ability to measure ¹⁴CO and ¹⁴CO₂ production rates simultaneously, following a single injection of labeled glycine. Examples are given to show that this continuous, in vivo, and easily performed method can give important information concerning heme catabolism. The technique should provide a unique source of information in the study of disease processes characterized by abnormal heme catabolism in man and other animals.

FOOTNOTES

¹ Presented in part at the 13th Annual Convention of the Society of Nuclear Medicine, Philadelphia, Pennsylvania, June, 1966. This work was supported in part by a National Heart Institute Post-Doctoral Fellowship.

² Post-Doctoral Research Fellow, Donner Laboratory, University of California, Berkeley.

³ Associate Research Physician, Donner Laboratory.

This article has been cited by other articles:

				J. R. Goldsmith and S. A. Landaw Carbon Monoxide and Human Health Science, December 20, 1968; 162(3860): 1352 - 1359. [PDF]